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Are the dyes specific for mitochondria and lysosomes How many mitochondria/lysosomes are there in a typical cell What is the distribution of their lengths/diameters Determine if you can see mitochondria round up and migrate toward the center of the cell, a stress response of the cell to non-ideal environmental conditions and prolonged observation Make sketches Bodipy phalloidin/actin stress fibers: After rinsing in HMEM, fix the cells in 4 mL 05% glutaraldehyde in HMEM for 15 min Dilute the dye 1:40 in the culture medium containing 01% Triton 100 detergent and incubate the cells at 37 C for 30 min Mount and examine after thorough rinsing in HMEM.

Unfortunately the two are often con ated or the importance of the latter ignored or downgraded Dowie (1999) argues passionately against use of risk He believes it confuses rather than elucidates It is too narrow He would prefer that the focus was on decisionmaking..

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Are the filaments you see individual actin filaments or filament bundles How are stress fibers oriented with respect to the long axis of the cell, cell borders, and vertices that represent points of cell attachment Estimate the amount of fluorescence associated with stress fibers vs unorganized filaments subjacent to the plasma membrane Prepare careful drawings Examination of double stained specimens If the microscope is equipped with two different filter sets for the same dye (for example, for examination of fluorescein, a filter set with a narrow band-pass emission filter and another set with a long-pass emission filter), it is useful to examine specimens double stained with fluorescein and rhodamine It is possible to directly examine the transmission profiles of the dichroic mirror-emission filter combination of a given filter set right at the microscope.

Table 12.2 Germ cell tumor cell types with associated tumor marker Cell type Dysgerminoma Endodermal sinus tumor Embryonal carcinoma Polyembryoma Choriocarcinoma Mixed germ cell Immature teratoma Tumor marker LDH, hCGa AFP, LDH hCG, AFP LDHa hCG, AFP hCG, LDHa AFPa LDHa AFPa , LDHa

Just switch the microscope to transmitted white light, mount a diffraction grating on the stage, close the condenser diaphragm down to a minimum, and examine the colors of the first order diffraction spectra with the aid of a Bertrand lens Examine all of the filter sets to determine the bands of wavelengths transmitted by the sets (1) Is there any bleed-through of fluorescence of one dye through the filter set intended for viewing the other dye (2) Is the amount of rhodamine bleed-through different for the two types of fluorescein filter sets Why In preparing to answer why bleed-through occurs, it is useful to compare transmission profiles of the various filters of the filter sets and to compare them with the excitation and emission.

Benign sex headache causes a sudden, severe headache which occurs at the moment of orgasm in men or women. The rst attack may be dif cult to distinguish from subarachnoid haemorrhage (which can, of course, occur at times of exertion). It is very reassuring if one discovers a history of previous attacks under similar circumstances. Happily, and unlike subarachnoid haemorrhage, benign sex headaches are not associated with loss of consciousness or vomiting. The attacks tend to disappear spontaneously but beta-adrenergic blockers can be a very effective preventive treatment.

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