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The person accessing the database is expected to know the names and meaning of all of the attributes and the expected ranges of attribute values Knowledge-based access may rely upon the concepts used to describe a discipline or a business An example is the emerging topic map ISO standard [11], which makes it possible to de ne terms that will be used in a business, and then map from these terms to the attribute names implemented within a local database In this case, the knowledge relationships that are exploited are logical relationships between semantic terms An example is the use of is a and has a logical relationships to de ne whether two terms are semantically equivalent or subordinate [12 15] The Grid in the data management matrix that interconnects the rectangles represents the interoperability mechanisms that make up the levels of abstraction.

If you preselect options in any of these palettes when no element is currently selected in the layout, they become the new default settings and are automatically applied to the next layout element you create.

Figure 13-26: Although similar to the standard Cell Property inspector, the Layout Cell Property inspector offers a different set of options.

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834 Primary Culture of DPSCs/SHED This isolation protocol is based on the ability of stem cells to adhere to culture dishes and form discrete colony clusters [Friedenstein, 1976; Friedenstein et al, 1970] To obtain colonies each derived from a single cell (ie, clones), it is very important to release DPSCs and SHED from their perivascular niche in the pulp tissue [Shi and Gronthos, 2003] Use a sterile scalpel blade and chop up the pulp into small segments Enzyme digestion is then required for harvesting putative stem cells from pulp tissue However, it may be harmful for the stem cells if enzyme digestion lasts for more than 1 h Each researcher should nd optimal conditions for the digestion Usually, it takes 30 60 min to fully digest well-minced pulp tissue.

It is recommended that the medium be changed 1 day after cell isolation; this minimizes the incidence of contamination However, when the risk of contamination is high, the medium can be changed 5 h after the isolation Cells isolated from dental pulp are usually seeded at 1 10 103 cells/cm2 for culture To evaluate their colony formation rate (CFU-F assay), a lower cell density (01 10 103 cells/cm2 ) is recommended in order to distinguish each single colony cluster Puri ed preparations of dental pulp stem cells can be obtained by immunoselection prior to culture [Shi and Gronthos, 2003] [see Protocol 82, Step (g)] Single-cell suspensions of enzyme-digested dental pulp tissue are obtained by passing the cells through a 70- m cell strainer.

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The lower row of the Grid represents a data handling system used to manage access to multiple storage systems [16] An example of such a system is the San Diego Supercomputer Center (SDSC) Storage Resource Broker (SRB) [17, 18] The system provides a uniform storage.

The cells are then incubated with primary antibodies reactive with DPSC, using STRO-1 (mouse anti-human MSC; IgM), CC9 (mouse anti-human CD146/MUC-18; IgG2a ), or 3G5 (mouse anti-human pericyte; IgM) [Shi and Gronthos, 2003] After this, the cells are washed with PBSA containing 1% bovine serum albumin (BSA) and then incubated with either sheep anti-mouse IgG-conjugated or rat anti-mouse IgM-conjugated magnetic Dynabeads Cells bound to beads are removed with the Dynal MPC -1 magnetic particle concentrator The STRO-1-, CD146-, or 3G5-positive cells are then seeded at 1 10 103 / cm2 in growth medium as described below [see Protocol 82, Step (h)]..

If a subpath is filled in and you want it to be transparent, or vice versa, click the compound path with the Direct Selection tool and then click the compound path whose direction you want to change and choose Object Paths Release Compound Path or press Option+Shift+ +8 or Ctrl+Alt+Shift+8 to separate the subpaths. Figure 16.23 shows how changing the direction of a subpath changes it from filled to transparent.

Points and picas are traditionally font-related measures. As such, they are typically used with font-related 1 CSS properties. However, because they both have absolute measures a point is equal to 72 of an inch and a pica is equal to 12 points you can use these metrics with any property that can take a length, width, or depth measure.

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