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In order to break away from the erroneous assumptions that are so widely popularised about serial criminals and psychological pro ling we need to develop a fully- edged scienti c discipline that will generate processes and theories for contributing to police investigations that have their roots rmly within empirical, scienti c psychology. In order to develop such a discipline one important foundation is to guard against a merely prurient fascination with crimes and criminals. Taking ctional accounts as the starting point for the consideration of psychological contributions to police investigations is therefore fraught with the risk of undermining this important foundation of scienti c work. Yet most academic accounts of such psychological contributions, under the heading of Offender Pro ling , start by citing these works of ction (e.g. Homant and Kennedy, 1998). The particular problem with such ction is that it almost invariably deals with the investigation of Serial Killers. These murderers provide such a simple icon of evil and depravity that they easily generate an apparently worthy foil for the hero of the story, the detective who solves the case. But in order to create an appealing ctional hero who will detect and uncover the villain, the hero s wit and virtues have to be emphasised by contrast with the person who kills over and over again. To emphasise the power of the hero (or more recently heroine) the killer has to be shown not only to

Transfer a cell-containing coverslip from a culture dish to a 35 mm dish containing HMEM (MEM culture medium plus 10 mM HEPES pH 7.2 and without serum). Buffering with HEPES (10 mM, pH 7.2) is necessary when handling and examining cultured cells in room air. Serum and phenol red should be omitted because their fluorescence decreases contrast. Remove the culture medium, add 2 mL HMEM and tilt gently to wash the medium over the cells. Pull off the medium with a Pasteur pipette and replace it with 2 mL HMEM containing a specific fluorescent dye at the appropriate concentration. Disperse the dye by gently rocking the culture dish. Incubate for the indicated time, retrieve and save the dye solution for reuse at a later time, rinse the coverslip 3 with 1 mL HMEM to remove any excess unbound dye, wipe off the back of the coverslip with a damp Kimwipe to remove cells and medium, and prepare a Vaseline mount as described in Figure 10-9. Draw off extra medium from the edge of the coverslip chamber with a filter paper and examine the preparations immediately. For each dye employed, describe the degree of staining specificity for organelles and explain why a dye might not appear to stain specifically. Also describe the intracellular abundance and morphological pattern exhibited by each type of labeled organelle. Make sketches and include scale bars on your drawings.

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13 ORGANIZATION OF THE BOOK In 2 we present the basic owgraph structures: series, parallel, and loop networks Using these as building blocks, more complex structures are created Work with owgraphs requires owgraph algebra, which manipulates probabilities and MGFs for accessing the waiting time distributions of interest Most statisticians have an aversion to MGFs, and in fact, most students of statistics treat MGFs as an annoyance that is only required knowledge for an exam Engineers, on the other hand, are quite comfortable with Laplace transforms, which are closely related to MGFs It turns out that owgraph algebra solves the waiting time distribution problem in the transformed world of MGFs One should think of the MGF in the same vein as polar coordinates or as any other transformation designed to make solutions more accessible.

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