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Microwave and electron cyclotron resonance CVD ( W / ECR CVD) Plasma is generated in a reactive gas mixture by a high-frequency electric field, such as generated by microwaves (Figure 233 (c)) or by electron cyclotron resonance (ECR) By using these methods, the coatings are very uniform over a large area (200 mm and more), smooth and of a high purity But the growth rate is very low, of the order of 01 m/h [40] By this process, large areas of uniform and homogeneous polycrystalline thin diamond films of a high quality can be obtained, predestined for electronic, optical and sensor applications Combustion synthesis (Oxy-acetylene torch and RF-high energy plasma torch) Combustion synthesis is another method in which the plasma is generated either by a chemical flame such as an oxygen acetylene torch, O2 C2H2 as depicted in Figure 233 (d) and Figure 233 (e) or by an HF torch (Figure 233 (f )) Both methods, chemical and physical, operate at very high temperatures, the chemical torch above 3,000 K and the plasma torches up to 8,000 K In both the cases the substrates to be coated need to be cooled When using oxygen acetylene torch, the deposition mode using a flame in the turbulent rather than in the laminar flow regime presents some advantages especially in what concerns the growth rate, which can be an order of magnitude greater In both modes, the coatings are irregular, non-homogeneous but relatively pure, depending on the purity of the gases Acetylene, C2H2, unfortunately cannot be easily cleaned economically to a high purity The growth rate can be orders of magnitude greater than obtained by other methods and is, for oxygen acetylene torch in the turbulent flame regime, typically 50 m/h

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Haploid Mapping (Tetrad Analysis)

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It should now be possible to describe exactly what type of crossover event produced each of the seven classes in table 6.7. Unfortunately, these two distances do not provide a unique determination of gene order. In gure 6.24, we see that two alternatives are possible: one has a map distance between the loci of 21.4 map units; the other has 4.6 map units between loci. How do we determine which of these is correct The simplest way is to calculate the a b distance using the unordered spore information. That is, the map distance is

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At this point, we recognize that the integral under the square root sign is equal to zero (see Example 47), because we are integrating a sinusoidal waveform over two periods Hence: I irms = = 0707I 2 where I is the peak value of the waveform i(t)

(Contd )

(1 2)118 1,000

Comments: The rms value of a sinusoidal signal is equal to 0707 times the peak value,

The preceding example illustrates how the rms value of a sinusoid is pro portional to its peak amplitude The factor of 0707 = 1/ 2 is a useful number to remember, since it applies to any sinusoidal signal It is not, however, generally applicable to signal waveforms other than sinusoids, as the Check Your Understanding exercises will illustrate

The eight random arrangements possible when a single crossover occurs between the a and b loci in Neurospora (see table 6.6). Circular arrows represent the rotation of a centromere from its position in the original con guration.

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46 Express the voltage v(t) = 1556 sin(377t + 60 ) in cosine form You should note that the radian frequency = 377 will recur very often, since 377 = 2 60; that is, 377 is the radian equivalent of the natural frequency of 60 cycles/second, which is the frequency of the electric power generated in North America 47 Compute the average value of the sawtooth waveform shown in Figure 422 48 Compute the average value of the shifted triangle wave shown in Figure 423

v (t) V 5 0 10 20 t (ms)

Since 6.2 map units is much closer to the a b distance expected if both loci are on the same side of the centromere, we accept alternative 2 in gure 6.24. A second way to choose between the alternatives in gure 6.24 is to nd out what happens to the b locus when a crossover occurs between the a locus and its centromere. If the order in alternative 1 is correct, crossovers between the a locus and its centromere should have no effect on the b locus; if 2 is correct, most of the crossovers that move the a locus in relation to its centromere should also move the b locus. Asci classes 4, 5, 6, and 7 include all the SDS patterns for the a locus. Of 168 asci, 150 (class 5) have similar SDS patterns for the b locus. Thus, 89% of the time, a crossover between the a locus and its centromere is also a crossover between the b locus and its centromere compelling evidence in favor of alternative 2. (What form would the data take if alternative 1 were correct ) In summary, mapping by tetrad analysis proceeds as follows. For both ordered and unordered spores, linkage is indicated by an excess of parental ditypes over nonparental ditypes. For unordered spores (yeast), the distance between two loci is one-half the number of

Fig 233: Six methods of diamond deposition are shown here [40, 41]: (a) A hot filament reactor, (b) a schematic

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