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HPRT TK . Eventually, the hybrid cells end up with random numbers of human chromosomes. There is one restriction: All cell lines selected are TK .This HAT method (using the HAT medium) not only selects for hybrid clones, but also localizes the TK gene to human chromosome 17, the one human chromosome found in every successful cell line.

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Note that the inductor now appears to behave like a complex frequency-dependent resistor, and that the magnitude of this complex resistor, L, is proportional to the signal frequency, Thus, an inductor will impede current ow in proportion to the sinusoidal frequency of the source signal This means that at low signal frequencies, an inductor acts somewhat like a short circuit, while at high frequencies it tends to behave more as an open circuit The Capacitor An analogous procedure may be followed to derive the equivalent result for a capacitor Beginning with the de ning relationships for the ideal capacitor, iC (t) = C 1 vC (t) = C dvC (t) dt iC (t ) dt

Map units between a locus and its centromere in organisms with ordered spores, such as Neurospora, can be calculated as

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A CBN tool possesses various characteristics, one of which is its extremely high hardness at room temperature (Knoop s hardness of 39 GPa), and it consists of single-crystal CBNs in a binder phase A range of CBN materials may be obtained determined by varying the CBN content, CBN grain size and binder phase materials According to Wentorf et al [43], a single-crystal cubic boron nitride is formed by conversion from a hexagonal boron nitride crystal It has the structure of zincblende, comprising two interpenetrating face centred cubic lattices, one of boron and the other of nitrogen Figure 240 shows the microstructures of the CBN materials used by Harris et al [44],

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Crossing over also occurs during mitosis, but at a much reduced rate. Somatic (mitotic) crossing over can be used to map loci. STUDY OBJECTIVE 3: To learn about analytical tech-

with iC = i and vC = vS in Figure 433, the capacitor current may be expressed as: iC (t) = C =C dvC (t) dt d (A cos t) dt (462)

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= C(A sin t) = CA cos( t + /2) so that, in phasor form, VS (j ) = A 0 I(j ) = CA /2 (463)

Human chromosomes can be mapped. Recombination distances can be established by pedigrees, and loci can be attributed to speci c chromosomes by synteny and assignment tests in hybrid cell lines.

The impedance of the ideal capacitor, ZC (j ), is therefore de ned as follows: VS (j ) 1 = /2 I(j ) C Impedance of a capacitor

Fig 240: Microstructures of the PCBN materials [44]

ZC (j ) =

PROBLEM 1: A homozygous claret (ca, claret eye color),

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where we have used the fact that 1/j = e j /2 = j Thus, the impedance of a capacitor is also a frequency-dependent complex quantity, with the impedance of the capacitor varying as an inverse function of frequency; and so a capacitor acts

curled (cu, upcurved wings), uted ( , creased wings) fruit y is crossed with a pure-breeding wild-type y. The F1 females are testcrossed with the following results:

when investigating the effect of temperature on the hardness of polycrystalline cubic boron nitride cutting tool materials Among all materials, Cubic Boron Nitride is known as the second hardest material second only to that of diamond It has excellent wear durability, which is also second only to diamond It also has a considerably high hot hardness, chemical inertness with respect to ferrous materials, good thermal resistance and a high coefficient of thermal conductivity, its cutting property under high temperature therefore being better than that of diamond Since it has a good thermal stability, it is unlikely to react chemically with the workpiece under high thermal conditions As a result, in manufacturing, CBN tools are mainly used in the processing of hard-to-machine materials Table 29 shows the comparison of properties of Polycrystalline Cubic Boron Nitride (PCBN) and other cutting tool materials Table 29 Comparison of properties of polycrystalline cubic boron nitride and other tool materials [44]

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