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where H is the enthalpy change associated with complex formation and R is the Universal Gas Constant. This means that measurements of uorescence quenching may be made under varying conditions of temperature and viscosity. In the case of solely dynamic quenching the slope of Stern-Volmer plots is expected to vary linearly with T / while in solely static quenching, its log varies linearly with 1/T (Figure 3.26). In practice, a particular uor/quencher pair may interact by a combination of these two mechanisms. For details of analysis of such complex situations literature in the bibliography at the end of this chapter should be consulted. Fluorescence spectra are strongly in uenced by exposure to solvent and/or quenchers present in the solvent. This fact underlies much of the practical usefulness of uorescence spectroscopy in biochemistry. For example, slight changes in the spatial position or solvent exposure of intrinsic protein uors such as tryptophan can result in either an increase or a decrease of quantum yield as well as qualitative changes in the spectrum such as a shift to longer wavelengths (a red shift). Fluorescence measurements may be performed at single emission wavelengths or, more informatively, may be acquired across the whole spectrum. Moreover, uorescence measurements may be made under steady-state conditions (i.e. at a single time) or may be time-resolved (i.e. measurements are taken at various times along a time-course). In order to give an indication of the versatility of this technique, we will now describe some of the main applications of uorescence in biochemistry.

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The traditional concept of energy homeostasis implies that weight gain develops as result of a chronic positive energy imbalance. According to a model proposed by Flatt (1988; 1995), the long-term stability of body weight requires not only that energy expenditure equals intake, but also that the relative proportions of fuels oxidized are the same as those consumed. Protein and carbohydrate stores in the body are limited, and they tend to be modulated by an autoregulatory process that allows their oxidation rate to increase in response to their own oxidation (Flatt, 1988; 1995). By contrast, fat oxidation is not tightly regulated by the fat stores, and fat oxidation rises only slowly as an adaptive response to an increase in dietary fat intake. The failure to adjust fat oxidation to a high-fat diet will lead to a depletion of the body s glycogen stores by increasing carbohydrate oxidation, and it is believed that this glycogen depletion may act as a signal to increase energy intake (Flatt, 1988). Human intervention trials have shown that a greater proportion of excess energy is stored as fat during high-fat overfeeding, compared with high-carbohydrate overfeeding, and that this is due to the minimal enhancement of fat oxidation and energy expenditure during the high-fat diet (Horton et al., 1995). Flatt and colleagues have argued that one of the mechanisms for adapting to a high-fat diet

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Data Centre Provider : provides the computing environment that hosts the content and the applications owned by the Information Providers Examples of such environments are video servers and massive server farms which implement the Internet service layer architecture (client-server, three tier architecture, dynamic content creation) for providing information services upon request For performance reasons such providers are directly connected to the Internet backbone or to access networks Since such computing environments can viewed as merely infrastructure, a Data Centre Provider may well be classi ed as offering infrastructure layer services Another use of data centres is to host points of presence of ISPs, BSPs, and other telecoms operators In this case, the data centre may play the role of a NAP and is called a telecom hotel .

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Characteristic x-rays are introduced into the spectrum of emitted x-rays when the high-speed electrons have sufficient energy to dislodge orbital electrons from the target material. Such collisions create shell vacancies that are filled by less tightly bound electrons, and in order to reach the lower energy state of the vacated shell these electrons must emit electromagnetic radiation. This radiation is characteristic of the target atoms, hence the term characteristic x-rays. The spikes in the spectrum shown in Figure 15-3 are the K-characteristic x-rays of tungsten, and are superimposed on the continuous spectrum of bremsstrahlung. The Ka1 (59.3 keV) and Ka2 (57.9 keV) characteristic x-rays occur when L-shell electrons from the LIII and LII subshells, respectively, fill K-shell vacancies. The Kb1 (keV) represents an M-shell to K-shell transition, and the Kb2 (69 keV) x-rays in tungsten occur from N-shell transitions. No characteristic x-rays are produced when the tube voltage is below 69 kVp in tungsten targets; at 80 kVp characteristic x-rays represent about 10% of the useful x-ray beam, and about 28% at 150 kVp. The relative contribution of characteristic radiation in tungsten decreases significantly above 150 kVp, and is a negligible fraction above about 300 kVp.

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